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S2 , n = 3). Each cell culture was performed at 37 °C in an atmosphere containing 5% CO 2 , and treated with various concentrations of drugs for 48 h. Cell viability was assessed using the MTS assay reagent (Promega). Data were analyzed using a nonlinear regression curve-fitting package in Prism 4 (GraphPad Software Inc.) (Fig.
S2 ). The drugs tested included: docetaxel, a mitotic inhibitor; irinotecan, a topoisomerase I inhibitor; 5-fluorouracil (5-FU), a thymidylate synthase inhibitor; sunitinib, a multi-receptor tyrosine kinase (RTK) inhibitor; tirapazamine, a hypoxia-activated prodrug; and FX11, a lactate dehydrogenase inhibitor. Radial organization of each cell on the spheroidal culture dish (IWAKI) conferred resistance to the most of drugs tested." width="100%" height="100%">
Journal: Scientific Reports
Article Title: Hyperpolarized [1- 13 C]pyruvate NMR spectroscopy reveals transition of tumor energy metabolism in microscale multicellular spheroids
doi: 10.1038/s41598-025-03454-1
Figure Lengend Snippet: IC 50 and IC 90 values (μM) of anti-tumor drugs for monolayer and spheroid-cultured cells in a 96-well format (Fig. S2 , n = 3). Each cell culture was performed at 37 °C in an atmosphere containing 5% CO 2 , and treated with various concentrations of drugs for 48 h. Cell viability was assessed using the MTS assay reagent (Promega). Data were analyzed using a nonlinear regression curve-fitting package in Prism 4 (GraphPad Software Inc.) (Fig. S2 ). The drugs tested included: docetaxel, a mitotic inhibitor; irinotecan, a topoisomerase I inhibitor; 5-fluorouracil (5-FU), a thymidylate synthase inhibitor; sunitinib, a multi-receptor tyrosine kinase (RTK) inhibitor; tirapazamine, a hypoxia-activated prodrug; and FX11, a lactate dehydrogenase inhibitor. Radial organization of each cell on the spheroidal culture dish (IWAKI) conferred resistance to the most of drugs tested.
Article Snippet: Cell viability in each drug concentration was plotted (Fig. ), and the sigmoidal dose–response curve was calculated using a nonlinear regression curve fitting package (sigmoidal dose–response) in Prism 4 (GraphPad Software Inc., La Jolla, CA).
Techniques: Cell Culture, MTS Assay, Software